Jan Buchholz, Jan Wolfgang Krieger, Gábor Mocsár, Balázs Kreith, Edoardo Charbon, György Vámosi, Udo Kebschull, Jörg Langowski
With the evolving technology in CMOS integration, new classes of 2D-imaging
detectors have recently become available. In particular, single photon
avalanche diode (SPAD) arrays allow detection of single photons at high
acquisition rates (\geq 100 kfps), which is about two orders of magnitude
higher than with currently available cameras. Here we demonstrate the use of a
SPAD array for imaging fluorescence correlation spectroscopy (imFCS), a tool to
create 2D maps of the dynamics of fluorescent molecules inside living cells.
Time-dependent fluorescence fluctuations, due to fluorophores entering and
leaving the observed pixels, are evaluated by means of autocorrelation
analysis. The multi-{\tau} correlation algorithm is an appropriate choice, as
it does not rely on the full data set to be held in memory. Thus, this
algorithm can be efficiently implemented in custom logic. We describe a new
implementation for massively parallel multi-{\tau} correlation hardware. Our
current implementation can calculate 1024 correlation functions at a resolution
of 10{\mu}s in real-time and therefore correlate real-time image streams from
high speed single photon cameras with thousands of pixels.
View original:
http://arxiv.org/abs/1112.1619
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